Parliamentary Private Secretary Selaine Saxby announced her resignation via Twitter.
Private Parliamentary Secretary Claire Coutinho and Private Parliamentary Secretary David Johnston also stepped down from their positions, as reported by Reuters.
Meanwhile, when asked about the possibility of British Prime Minister Boris Johnson facing another no-confidence vote, the PM's spokesperson said that Johnson was confident that he still had the support of his backbench MPs. "There will be further appointments over the coming days," she added.
Market reaction
The GBP/USD pair stays under heavy bearish pressure on Wednesday and was last seen losing 0.6% on the day at 1.1885.